The effect of swimming training, cell and laser on the expression of genes involved in autophagy (LC3 and Beclin-1) in azoospermia mice

Background and Aim: Infertility in men is 10-20% of cases due to azoospermia. The aim of this study was to investigate the effect of swimming training, cell and laser on the expression of genes involved in autophagy (LC3 and Beclin-1) in azoospermia mice. Methods: 30 rats 6 to 8 weeks of age were randomly selected from Tehran Laboratory Animal Research and Propagation Center, and then injected intraperitoneally with a 40 mg dose of busulfan for each rat and an azoospermia model was created. One month after induction of the model in each group, the mice were subdivided into: patient control, healthy control, patient control + laser, patient control + exercise, patient control group + cell and patient control group + Cell + Laser + Exercise (One month after the creation of the one-time model of stem cells transplanted in the area of the esophageal ducts, one million cells were transplanted for each mouse, then a week after transplantation of the low-power laser cell With a wavelength of 8.632 nm, a power of 10 mW, and 3 joules of energy, it was applied three times throughout the study period, once a week, and after wound healing, the cell transplant site on the abdomen was administered daily for 30 days. They swam 30 minutes a day, 5 days a week, for 8 weeks. Results: The results showed that induction of azoospermia model significantly increased the expression of LC3 (P≤0/01) and Beclin-1 (P≤0/01) genes of testicular tissue compared to the control group. Swimming in combination with cells and laser reduced LC3 (P≤0/05) and Beclin-1 (P≤0/05) and inhibited autophagy in azoospermia mice. Conclusion: Swimming training combined with cell therapy and laser therapy with inhibited autophagy may exert its protective effect on mice in the azoospermia model and cause the mice to become fertile.